dc.contributor.author |
Isaja, Luciana |
|
dc.contributor.author |
Ferriol Laffouillere, Sofía Luján |
|
dc.contributor.author |
Mucci, Sofía |
|
dc.contributor.author |
Rodríguez Varela, María Soledad |
|
dc.contributor.author |
Romorini, Leonardo |
|
dc.date.accessioned |
2021-11-02T17:20:02Z |
|
dc.date.available |
2021-11-02T17:20:02Z |
|
dc.date.issued |
2021-10-06 |
|
dc.identifier.citation |
Isaja L, Ferriol Laffouillere SL, Mucci S, Rodríguez Varela MS, Romorini L. Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions. Methods Mol Biol. 2021 Oct 6. doi: 10.1007/7651_2021_440 |
es_ES |
dc.identifier.uri |
https://doi.org/10.1007/7651_2021_440 |
|
dc.identifier.uri |
https://repositorio.fleni.org.ar/xmlui/handle/123456789/613 |
|
dc.description.abstract |
Human embryonic stem cells (hESCs) can differentiate into any cell lineage (pluripotency potential) derived from the three germ layers: ectoderm, mesoderm, and endoderm. Pluripotency is usually demonstrated in vitro by spontaneous differentiation of hESCs grown on a monolayer of feeder-cells using an embryoid bodies (EBs)-based method. However, currently hESCs are grown mostly using fully defined media in the absence of a feeder layer. Here we describe a EBs-based protocol that allows multilineage differentiation of hESCs and human induced pluripotent stem cells (hiPSCs) grown on feeder-free conditions. |
es_ES |
dc.language.iso |
eng |
es_ES |
dc.publisher |
Springer |
es_ES |
dc.rights |
info:eu-repo/semantics/openAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/2.5/ar/ |
|
dc.subject |
Embryoid Bodies |
es_ES |
dc.subject |
Cuerpos Embrioides |
es_ES |
dc.subject |
Human Embryonic Stem Cells |
es_ES |
dc.subject |
Células Madre Embrionarias Humanas |
es_ES |
dc.subject |
Induced Pluripotent Stem Cells |
es_ES |
dc.subject |
Células Madre Pluripotentes Inducidas |
es_ES |
dc.title |
Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions |
es_ES |
dc.type |
info:eu-repo/semantics/article |
es_ES |
dc.type |
info:eu-repo/semantics/publishedVersion |
|
dc.description.fil |
Fil: Isaja, Luciana. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina. |
|
dc.description.fil |
Fil: Ferriol Laffouillere, Sofía Luján. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina. |
|
dc.description.fil |
Fil: Mucci, Sofía. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina. |
|
dc.description.fil |
Fil: Rodríguez Varela, María Soledad. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina. |
|
dc.description.fil |
Fil: Romorini, Leonardo. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina. |
|
dc.relation.ispartofCOUNTRY |
Estados Unidos |
|
dc.relation.ispartofCITY |
Totowa |
|
dc.relation.ispartofTITLE |
Methods in molecular biology |
|
dc.relation.ispartofISSN |
1940-6029 |
|
dc.type.snrd |
info:ar-repo/semantics/artículo |
es_ES |