Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions

Isaja, Luciana; Ferriol Laffouillere, Sofía Luján; Mucci, Sofía; Rodríguez Varela, María Soledad; Romorini, Leonardo

dc.contributor.author	Isaja, Luciana
dc.contributor.author	Ferriol Laffouillere, Sofía Luján
dc.contributor.author	Mucci, Sofía
dc.contributor.author	Rodríguez Varela, María Soledad
dc.contributor.author	Romorini, Leonardo
dc.date.accessioned	2021-11-02T17:20:02Z
dc.date.available	2021-11-02T17:20:02Z
dc.date.issued	2021-10-06
dc.identifier.citation	Isaja L, Ferriol Laffouillere SL, Mucci S, Rodríguez Varela MS, Romorini L. Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions. Methods Mol Biol. 2021 Oct 6. doi: 10.1007/7651_2021_440	es_ES
dc.identifier.uri	https://doi.org/10.1007/7651_2021_440
dc.identifier.uri	https://repositorio.fleni.org.ar/xmlui/handle/123456789/613
dc.description.abstract	Human embryonic stem cells (hESCs) can differentiate into any cell lineage (pluripotency potential) derived from the three germ layers: ectoderm, mesoderm, and endoderm. Pluripotency is usually demonstrated in vitro by spontaneous differentiation of hESCs grown on a monolayer of feeder-cells using an embryoid bodies (EBs)-based method. However, currently hESCs are grown mostly using fully defined media in the absence of a feeder layer. Here we describe a EBs-based protocol that allows multilineage differentiation of hESCs and human induced pluripotent stem cells (hiPSCs) grown on feeder-free conditions.	es_ES
dc.language.iso	eng	es_ES
dc.publisher	Springer	es_ES
dc.rights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/2.5/ar/
dc.subject	Embryoid Bodies	es_ES
dc.subject	Cuerpos Embrioides	es_ES
dc.subject	Human Embryonic Stem Cells	es_ES
dc.subject	Células Madre Embrionarias Humanas	es_ES
dc.subject	Induced Pluripotent Stem Cells	es_ES
dc.subject	Células Madre Pluripotentes Inducidas	es_ES
dc.title	Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.type	info:eu-repo/semantics/publishedVersion
dc.description.fil	Fil: Isaja, Luciana. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina.
dc.description.fil	Fil: Ferriol Laffouillere, Sofía Luján. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina.
dc.description.fil	Fil: Mucci, Sofía. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina.
dc.description.fil	Fil: Rodríguez Varela, María Soledad. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina.
dc.description.fil	Fil: Romorini, Leonardo. Fleni. Laboratorios de Investigación Aplicada en Neurociencias; Argentina.
dc.relation.ispartofCOUNTRY	Estados Unidos
dc.relation.ispartofCITY	Totowa
dc.relation.ispartofTITLE	Methods in molecular biology
dc.relation.ispartofISSN	1940-6029
dc.type.snrd	info:ar-repo/semantics/artículo	es_ES