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Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS

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dc.contributor.author Gimenez, Rocio
dc.contributor.author Pérez-Sosa, Camilo
dc.contributor.author Bourguignon, Natalia
dc.contributor.author Miriuka, Santiago Gabriel
dc.contributor.author Bhansali, Shekhar
dc.contributor.author Arroyo, Carlos R.
dc.contributor.author Debut, Alexis
dc.contributor.author Lerner, Betiana
dc.contributor.author Pérez, Maximiliano S.
dc.date.accessioned 2023-01-12T12:24:19Z
dc.date.available 2023-01-12T12:24:19Z
dc.date.issued 2022-10-08
dc.identifier.citation Gimenez R, Pérez-Sosa C, Bourguignon N, Miriuka S, Bhansali S, Arroyo CR, Debut A, Lerner B, Pérez MS. Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS. Biomimetics (Basel). 2022 Oct 8;7(4):155. doi: 10.3390/biomimetics7040155. es_ES
dc.identifier.uri https://doi.org/10.3390/biomimetics7040155
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/772
dc.description.abstract Microcontact printing using PDMS embossing tools and its variations have aroused the interest of a wide spectrum of research fields, hence the feasibility of defining micro and nanoscale patterns. In this work, we have proposed and demonstrated a novel lithography method based on grayscale patterns printed in a flexographic photopolymer mold and transferred to epoxy resin and a single PDMS stamp to obtain different microprint pattern structures. The geometry of the patterns can be modified by adjusting the layout and grayscale of the stamp patterns. The functionality of this contact printing methodology was validated by generating human induced pluripotent stem cells (hiPSC) patterns. These specific micropatterns can be very useful for achieving complex differentiation in cell lines such as hiPSC. Microfabrication through the new technique provides a promising alternative to conventional lithography for constructing complex aligned surfaces; these structures could be used as components of biological patterns or microfluidic devices. es_ES
dc.language.iso eng es_ES
dc.publisher MDPI AG es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Biomimetics es_ES
dc.subject Biomimética es_ES
dc.subject Bionics es_ES
dc.subject Biónica es_ES
dc.title Simple Microcontact Printing Technique to Obtain Cell Patterns by Lithography Using Grayscale, Photopolymer Flexographic Mold, and PDMS es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Gimenez, Rocio. Universidad Tecnológica Nacional; Argentina.
dc.description.fil Fil: Pérez-Sosa, Camilo. Universidad Tecnológica Nacional; Argentina.
dc.description.fil Fil: Bourguignon, Natalia. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados Unidos.
dc.description.fil Fil: Miriuka, Santiago Gabriel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Bhansali, Shekhar. Florida International University; Estados Unidos.
dc.description.fil Fil: Arroyo, Carlos R. Universidad de las Fuerzas Armadas ESPE; Ecuador.
dc.description.fil Fil: Debut, Alexis. Universidad de las Fuerzas Armadas ESPE; Ecuador.
dc.description.fil Fil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados Unidos. Hermann-Herder-Straße 4; Alemania.
dc.description.fil Fil: Pérez, Maximiliano S. Fil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Florida International University; Estados Unidos. Hermann-Herder-Straße 4; Alemania.
dc.relation.ispartofVOLUME 7
dc.relation.ispartofNUMBER 4
dc.relation.ispartofPAGINATION 155
dc.relation.ispartofCOUNTRY Suiza
dc.relation.ispartofCITY Basilea
dc.relation.ispartofTITLE Biomimetics
dc.relation.ispartofISSN 2313-7673
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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