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Browsing by Author "Waisman, Ariel"

Browsing by Author "Waisman, Ariel"

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  • 188 MicroRNA characterization in equine induced pluripotent stem cells 
    Moro, Lucía Natalia; Amín, Guadalupe; Furmento, Verónica Alejandra; Waisman, Ariel; Neiman, Gabriel; La Greca, Alejandro; Santín Velazque, Natalia Lucía; Luzzani, Carlos Daniel; Sevlever, Gustavo Emilio; Vichera, Gabriel; Miriuka, Santiago Gabriel (Csiro Publishing, 2018-12-03)
    Cell reprogramming has been well described in mouse and human cells. The expression of specific microRNAs has demonstrated to be essential for pluripotent maintenance and cell differentiation, but not much information is ...
  • A critical pathway in human development: C19MC micrornas regulates FGF2 response in a model of human pluripotent stem cells cardiac differentiation 
    Möbbs, Alan Miqueas; Garate, Ximena; Scarafía, María Agustina; Colli, Carolina; Moro, Lucía Natalia; Luzzani, Carlos Daniel; Waisman, Ariel; La Greca, Alejandro; Miriuka, Santiago Gabriel (Fundación Revista Medicina, 2021-11)
  • Analysis of the expression of PIWI-interacting RNAs during cardiac differentiation of human pluripotent stem cells 
    La Greca, Alejandro; Scarafía, María Agustina; Hernández Cañás, María Clara; Pérez, Nelba; Castañeda, Sheila; Colli, Carolina; Miqueas Möbbs, Alan; Santín Velazque, Natalia Lucía; Neiman, Gabriel; Garate, Ximena; Aban, Cyntia; Waisman, Ariel; Moro, Lucía Natalia; Sevlever, Gustavo Emilio; Luzzani, Carlos Daniel; Miriuka, Santiago Gabriel (bioRxiv, 2019-05-21)
    El artículo es un preprint que aún no ha pasado por revisión por pares PIWI-interacting RNAs (piRNAs) are a class of non-coding RNAs initially thought to be restricted almost exclusively to germ line cells. In recent ...
  • Automatic and unbiased segmentation and quantification of myofibers in skeletal muscle 
    Waisman, Ariel; Norris, Alessandra; Costa, Martín Elías; Kopinke, Daniel (Nature Publishing Group, 2021-01-22)
    Skeletal muscle has the remarkable ability to regenerate. However, with age and disease muscle strength and function decline. Myofiber size, which is affected by injury and disease, is a critical measurement to assess ...
  • Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency 
    Waisman, Ariel; Sevlever, Federico; Costa, Martín Elías; Cosentino, María Soledad; Miriuka, Santiago Gabriel; Ventura, Alejandra C.; Guberman, Alejandra S. (Nature Publishing Group, 2019-05-29)
    Mouse embryonic stem cells (mESCs) can be maintained as homogeneous populations in the ground state of pluripotency. Release from this state in minimal conditions allows to obtain cells that resemble those of the early ...
  • celldeath: A tool for detection of cell death in transmitted light microscopy images by deep learning-based visual recognition 
    La Greca, Alejandro; Pérez, Nelba; Castañeda, Sheila; Milone, Paula Melania; Scarafía, María Agustina; Möbbs, Alan Miqueas; Waisman, Ariel; Moro, Lucía Natalia; Sevlever, Gustavo Emilio; Luzzani, Carlos Daniel; Miriuka, Santiago Gabriel (Public Library of Science, 2021-06-24)
    Cell death experiments are routinely done in many labs around the world, these experiments are the backbone of many assays for drug development. Cell death detection is usually performed in many ways, and requires time and ...
  • Deep Learning Neural Networks Highly Predict Very Early Onset of Pluripotent Stem Cell Differentiation 
    Waisman, Ariel; La Greca, Alejandro; Möbbs, Alan Miqueas; Scarafía, María Agustina; Santín Velazque, Natalia Lucía; Neiman, Gabriel; Moro, Lucía Natalia; Luzzani, Carlos Daniel; Sevlever, Gustavo Emilio; Guberman, Alejandra S.; Miriuka, Santiago Gabriel (Elsevier, 2019-04-09)
    Deep learning is a significant step forward for developing autonomous tasks. One of its branches, computer vision, allows image recognition with high accuracy thanks to the use of convolutional neural networks (CNNs). Our ...
  • Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT 
    Aban, Cyntia; Lombardi, Antonella; Neiman, Gabriel; Biani, María Celeste; La Greca, Alejandro; Waisman, Ariel; Moro, Lucía Natalia; Sevlever, Gustavo Emilio; Miriuka, Santiago Gabriel; Luzzani, Carlos Daniel (Nature Publishing Group, 2021-01-21)
    Epithelial to mesenchymal transition (EMT) is a critical cellular process that has been well characterized during embryonic development and cancer metastasis and it also is implicated in several physiological and pathological ...
  • Extraction-free protocol combining proteinase K and heat inactivation for detection of SARS-CoV-2 by RT-qPCR 
    Genoud, Valeria; Stortz, Martin; Waisman, Ariel; Berardino, Bruno G.; Verneri, Paula; Dansey, Virginia; Salvatori, Melina; Remes Lenicov, Federico; Levi, Valeria (PLoS One, 2021-02-26)
    Real-time reverse transcription PCR (RT-qPCR) is the gold-standard technique for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in nasopharyngeal swabs specimens. The analysis by RT-qPCR usually ...
  • Integrin alpha-5 subunit is critical for the early stages of human pluripotent stem cell cardiac differentiation 
    Neiman, Gabriel; Scarafía, María Agustina; La Greca, Alejandro; Santín Velazque, Natalia Lucía; Garate, Ximena; Waisman, Ariel; Möbbs, Alan Miqueas; Kasai-Brunswick, Tais Hanae; Mesquita, Fernanda C.P.; Martire-Greco, Daiana; Moro, Lucía Natalia; Luzzani, Carlos Daniel; Bastos Carvalho, Adriana; Sevlever, Gustavo Emilio; Campos de Carvalho, Antonio C.; Guberman, Alejandra S.; Miriuka, Santiago Gabriel (Nature Publishing Group, 2019-12-02)
    The stem cell niche has a strong influence in the differentiation potential of human pluripotent stem cells with integrins playing a major role in communicating cells with the extracellular environment. However, it is not ...
  • Kat6b Modulates Oct4 and Nanog Binding to Chromatin in Embryonic Stem Cells and Is Required for Efficient Neural Differentiation 
    Cosentino, María Soledad; Oses, Camila; Vázquez Echegaray, Camila; Solari, Claudia; Waisman, Ariel; Álvarez, Yanina; Petrone, María Victoria; Francia, Marcos; Schultz, Marcelo; Sevlever, Gustavo Emilio; Miriuka, Santiago Gabriel; Levi, Valeria; Guberman, Alejandra S. (Academic Press, 2019-03-15)
    Chromatin remodeling is fundamental for the dynamical changes in transcriptional programs that occur during development and stem cell differentiation. The histone acetyltransferase Kat6b is relevant for neurogenesis in ...
  • PIWI-interacting RNAs are differentially expressed during cardiac differentiation of human pluripotent stem cells 
    La Greca, Alejandro; Scarafía, María Agustina; Hernández Cañás, María Clara; Pérez, Nelba; Castañeda, Sheila; Colli, Carolina; Miqueas Möbbs, Alan; Santín Velazque, Natalia Lucía; Neiman, Gabriel; Garate, Ximena; Aban, Cyntia; Waisman, Ariel; Moro, Lucía Natalia; Sevlever, Gustavo Emilio; Luzzani, Carlos Daniel; Miriuka, Santiago Gabriel (Public Library of Science, 2020-05-05)
    PIWI-interacting RNAs (piRNAs) are a class of non-coding RNAs initially thought to be restricted exclusively to germline cells. In recent years, accumulating evidence has demonstrated that piRNAs are actually expressed in ...
  • The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells 
    Solari, Claudia; Petrone, María Victoria; Toro, Ayelén; Vázquez Echegaray, Camila; Cosentino, María Soledad; Waisman, Ariel; Francia, Marcos; Barañao, Lino; Miriuka, Santiago Gabriel; Guberman, Alejandra S. (Biomed Central, 2019-07-01)
    OBJECTIVE: Redox homeostasis maintenance is essential to bring about cellular functions. Particularly, embryonic stem cells (ESCs) have high fidelity mechanisms for DNA repair, high activity of different antioxidant enzymes ...
  • Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system 
    Pérez-Sosa, Camilo; Sanluis-Verdes, Anahí; Waisman, Ariel; Lombardi, Antonella; Rosero, Gustavo; La Greca, Alejandro; Bhansali, Shekhar; Bourguignon, Natalia; Luzzani, Carlos Daniel; Pérez, Maximiliano S.; Miriuka, Santiago Gabriel; Lerner, Betiana (Royal Society Publishing, 2022-01-12)
    Microfluidic tools have recently made possible many advances in biological and biomedical research. Research in fields such as physics, engineering, chemistry and biology have combined to produce innovation in microfluidics ...
  • SUMO conjugation susceptibility of Akt/protein kinase B affects the expression of the pluripotency transcription factor Nanog in embryonic stem cells 
    Francia, Marcos; Stortz, Martin; Vazquez Echegaray, Camila; Oses, Camila; Verneri, Paula; Petrone, María Victoria; Toro, Ayelén; Waisman, Ariel; Miriuka, Santiago Gabriel; Cosentino, María Soledad; Levi, Valeria; Guberman, Alejandra S. (Public Library of Science, 2021-07-09)
    Akt/PKB is a kinase involved in the regulation of a wide variety of cell processes. Its activity is modulated by diverse post-translational modifications (PTMs). Particularly, conjugation of the small ubiquitin-related ...

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