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Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels.

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dc.contributor.author Pérez Sosa, Camilo
dc.contributor.author Pérez, Maximiliano S.
dc.contributor.author Vallejo Janeta, Alexander Paolo
dc.contributor.author Bhansali, Shekhar
dc.contributor.author Miriuka, Santiago Gabriel
dc.contributor.author Lerner, Betiana
dc.date.accessioned 2024-04-23T13:23:58Z
dc.date.available 2024-04-23T13:23:58Z
dc.date.issued 2024-02-19
dc.identifier.citation Pérez-Sosa C, Pérez MS, Vallejo-Janeta AP, Bhansali S, Miriuka S, Lerner B. Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels. Micromachines (Basel). 2024 Mar 19;15(3):413. doi: 10.3390/mi15030413. es_ES
dc.identifier.uri https://doi.org/10.3390/mi15030413
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/1089
dc.description.abstract Gene editing tools have triggered a revolutionary transformation in the realms of cellular and molecular physiology, serving as a fundamental cornerstone for the evolution of disease models and assays in cell culture reactions, marked by various enhancements. Concurrently, microfluidics has emerged over recent decades as a versatile technology capable of elevating performance and reducing costs in daily experiments across diverse scientific disciplines, with a pronounced impact on cell biology. The amalgamation of these groundbreaking techniques holds the potential to amplify the generation of stable cell lines and the production of extracellular matrix hydrogels. These hydrogels, assuming a pivotal role in isolating cells at the single-cell level, facilitate a myriad of analyses. This study presents a novel method that seamlessly integrates CRISPR-Cas9 gene editing techniques with single-cell isolation methods in induced pluripotent stem cell (hiPSC) lines, utilizing the combined power of droplets and hydrogels. This innovative approach is designed to optimize clonal selection, thereby concurrently reducing costs and the time required for generating a stable genetically modified cell line. By bridging the advancements in gene editing and microfluidic technologies, our approach not only holds significant promise for the development of disease models and assays but also addresses the crucial need for efficient single-cell isolation. This integration contributes to streamlining processes, making it a transformative method with implications for enhancing the efficiency and cost-effectiveness of stable cell line generation. As we navigate the intersection of gene editing and microfluidics, our study marks a significant stride toward innovative methodologies in the dynamic landscape of cellular and molecular physiology research. es_ES
dc.language.iso eng es_ES
dc.publisher MDPI es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.subject Microfluídica es_ES
dc.subject Microfluidics es_ES
dc.subject Análisis de la Célula Individual es_ES
dc.subject Single-Cell Analysis es_ES
dc.subject Proteína 9 Asociada a CRISPR es_ES
dc.subject CRISPR-Associated Protein 9 es_ES
dc.subject Robótica
dc.subject Robotics
dc.subject LIAN
dc.title Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels. es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Miriuka, Santiago Gabriel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.relation.ispartofVOLUME 15
dc.relation.ispartofNUMBER 3
dc.relation.ispartofPAGINATION 413.
dc.relation.ispartofCOUNTRY Suiza
dc.relation.ispartofCITY Basilea
dc.relation.ispartofISSN 2072-666X
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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