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dc.contributor.author | Neiman, Gabriel | |
dc.contributor.author | Scarafía, María Agustina | |
dc.contributor.author | La Greca, Alejandro | |
dc.contributor.author | Santín Velazque, Natalia Lucía | |
dc.contributor.author | Garate, Ximena | |
dc.contributor.author | Waisman, Ariel | |
dc.contributor.author | Möbbs, Alan Miqueas | |
dc.contributor.author | Kasai-Brunswick, Tais Hanae | |
dc.contributor.author | Mesquita, Fernanda C.P. | |
dc.contributor.author | Martire-Greco, Daiana | |
dc.contributor.author | Moro, Lucía Natalia | |
dc.contributor.author | Luzzani, Carlos Daniel | |
dc.contributor.author | Bastos Carvalho, Adriana | |
dc.contributor.author | Sevlever, Gustavo Emilio | |
dc.contributor.author | Campos de Carvalho, Antonio C. | |
dc.contributor.author | Guberman, Alejandra S. | |
dc.contributor.author | Miriuka, Santiago Gabriel | |
dc.date.accessioned | 2019-12-10T18:04:38Z | |
dc.date.available | 2019-12-10T18:04:38Z | |
dc.date.issued | 2019-12-02 | |
dc.identifier.citation | Neiman G, Scarafía MA, La Greca A, et al. Integrin alpha-5 subunit is critical for the early stages of human pluripotent stem cell cardiac differentiation. Sci Rep. 2019;9(1):18077. doi:10.1038/s41598-019-54352-2 | en_US |
dc.identifier.uri | https://doi.org/10.1038/s41598-019-54352-2 | |
dc.identifier.uri | https://repositorio.fleni.org.ar/handle/123456789/126 | |
dc.description.abstract | The stem cell niche has a strong influence in the differentiation potential of human pluripotent stem cells with integrins playing a major role in communicating cells with the extracellular environment. However, it is not well understood how interactions between integrins and the extracellular matrix are involved in cardiac stem cell differentiation. To evaluate this, we performed a profile of integrins expression in two stages of cardiac differentiation: mesodermal progenitors and cardiomyocytes. We found an active regulation of the expression of different integrins during cardiac differentiation. In particular, integrin α5 subunit showed an increased expression in mesodermal progenitors, and a significant downregulation in cardiomyocytes. To analyze the effect of α5 subunit, we modified its expression by using a CRISPRi technique. After its downregulation, a significant impairment in the process of epithelial-to-mesenchymal transition was seen. Early mesoderm development was significantly affected due to a downregulation of key genes such as T Brachyury and TBX6. Furthermore, we observed that repression of integrin α5 during early stages led to a reduction in cardiomyocyte differentiation and impaired contractility. In summary, our results showed the link between changes in cell identity with the regulation of integrin α5 expression through the alteration of early stages of mesoderm commitment. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.subject | Genes, Reporter | en_US |
dc.subject | Genes Reporteros | en_US |
dc.subject | Stem Cell Niche | en_US |
dc.subject | Nicho de Células Madre | en_US |
dc.subject | Stem Cell Differentiation | en_US |
dc.subject | Diferenciación de Células Madre | en_US |
dc.subject | Integrin alpha5 | en_US |
dc.subject | Integrina alfa5 | en_US |
dc.title | Integrin alpha-5 subunit is critical for the early stages of human pluripotent stem cell cardiac differentiation | en_US |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | info:eu-repo/semantics/article | en_US |
dc.description.fil | Fil: Neiman, Gabriel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Scarafía, María Agustina. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: La Greca, Alejandro. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Santín Velazque, Natalia Lucía. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Garate, Ximena. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Waisman, Ariel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Möbbs, Alan Miqueas. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Kasai-Brunswick, Tais Hanae. Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho; Brasil. | |
dc.description.fil | Fil: Mesquita, Fernanda C.P. Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho; Brasil. | |
dc.description.fil | Fil: Martire-Greco, Daiana. Academia Nacional de Medicina; Argentina. | |
dc.description.fil | Fil: Moro, Lucía Natalia. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Luzzani, Carlos Daniel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Bastos Carvalho, Adriana. Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho; Brasil. | |
dc.description.fil | Fil: Sevlever, Gustavo Emilio. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Campos de Carvalho, Antonio C. Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho; Brasil. | |
dc.description.fil | Fil: Guberman, Alejandra S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Regulación Génica en Células Madre; Argentina. | |
dc.description.fil | Fil: Miriuka, Santiago G. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.relation.ispartofVOLUME | 9 | |
dc.relation.ispartofNUMBER | 1 | |
dc.relation.ispartofCOUNTRY | Reino Unido | |
dc.relation.ispartofCITY | London | |
dc.relation.ispartofTITLE | Scientific reports | |
dc.relation.ispartofISSN | 2045-2322 | |
dc.type.snrd | info:ar-repo/semantics/artículo | es_ES |