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Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency

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dc.contributor.author Waisman, Ariel
dc.contributor.author Sevlever, Federico
dc.contributor.author Costa, Martín Elías
dc.contributor.author Cosentino, María Soledad
dc.contributor.author Miriuka, Santiago Gabriel
dc.contributor.author Ventura, Alejandra C.
dc.contributor.author Guberman, Alejandra S.
dc.date.accessioned 2020-10-14T13:39:10Z
dc.date.available 2020-10-14T13:39:10Z
dc.date.issued 2019-05-29
dc.identifier.citation Waisman, A., Sevlever, F., Costa, M.E., Cosentino, M.S., Miriuka, S.G., Ventura, A.C., Guberman, A.S. Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency. Scientific Reports 2019 May 29;9(1):8051. doi: 10.1038/s41598-019-44537-0. en_US
dc.identifier.uri https://doi.org/10.1038/s41598-019-44537-0
dc.identifier.uri https://repositorio.fleni.org.ar/handle/123456789/202
dc.description.abstract Mouse embryonic stem cells (mESCs) can be maintained as homogeneous populations in the ground state of pluripotency. Release from this state in minimal conditions allows to obtain cells that resemble those of the early post-implantation epiblast, providing an important developmental model to study cell identity transitions. However, the cell cycle dynamics of mESCs in the ground state and during its dissolution have not been extensively studied. By performing live imaging experiments of mESCs bearing cell cycle reporters, we show here that cells in the pluripotent ground state display a cell cycle structure comparable to the reported for mESCs in serum-based media. Upon release from self-renewal, the cell cycle is rapidly accelerated by a reduction in the length of the G1 phase and of the S/G2/M phases, causing an increased proliferation rate. Analysis of cell lineages indicates that cell cycle variables of sister cells are highly correlated, suggesting the existence of inherited cell cycle regulators from the parental cell. Together with a major morphological reconfiguration upon differentiation, our findings support a correlation between this in vitro model and early embryonic events. en_US
dc.language.iso eng en_US
dc.publisher Nature Publishing Group en_US
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Data Mining en_US
dc.subject Embryonic Stem Cells en_US
dc.subject Minería de Datos en_US
dc.subject Células Madre Embrionarias en_US
dc.title Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency en_US
dc.type info:eu-repo/semantics/publishedVersion
dc.type info:eu-repo/semantics/article en_US
dc.description.fil Fil: Waisman, Ariel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica. Laboratorio de Regulación Génica en Células Madre; Argentina. Fleni. Laboratorio de Investigación de Aplicación a Neurociencias; Argentina.
dc.description.fil Fil: Sevlever, Federico. Universidad de Buenos Aires, Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina.
dc.description.fil Fil: Costa, Martín Elías. Universidad de Buenos Aires; Argentina.
dc.description.fil Fil: Cosentino, María Soledad. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica. Laboratorio de Regulación Génica en Células Madre; Argentina.
dc.description.fil Fil: Miriuka, Santiago Gabriel. Fleni. Laboratorio de Investigación de Aplicación a Neurociencias; Argentina.
dc.description.fil Fil: Ventura, Alejandra C. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina.
dc.description.fil Fil: Guberman, Alejandra S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica. Laboratorio de Regulación Génica en Células Madre; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología y Biología Molecular y Celular; Argentina.
dc.relation.ispartofVOLUME 9
dc.relation.ispartofNUMBER 1
dc.relation.ispartofPAGINATION 8051
dc.relation.ispartofCOUNTRY Alemania
dc.relation.ispartofCITY Berlin
dc.relation.ispartofTITLE Scientific reports
dc.relation.ispartofISSN 2045-2322
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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