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Systemic Ghrelin Treatment Induces Rapid, Transient, and Asymmetric Changes in the Metabolic Activity of the Mouse Brain

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dc.contributor.author de Francesco, Pablo Nicolás
dc.contributor.author Fernández, Gimena
dc.contributor.author Uriarte, Maia
dc.contributor.author Urrutia, Leandro
dc.contributor.author Ponce de León, Magdalena
dc.contributor.author Fehrentz, Jean-Alain
dc.contributor.author Falasco, Germán
dc.contributor.author Perello, Mario
dc.date.accessioned 2023-09-08T12:43:30Z
dc.date.available 2023-09-08T12:43:30Z
dc.date.issued 2022-07-29
dc.identifier.citation De Francesco PN, Fernandez G, Uriarte M, Urrutia L, Ponce de León M, Fehrentz JA, Falasco G, Perello M. Systemic Ghrelin Treatment Induces Rapid, Transient, and Asymmetric Changes in the Metabolic Activity of the Mouse Brain. Neuroendocrinology. 2023;113(1):64-79. doi: 10.1159/000526245. Epub 2022 Jul 29. es_ES
dc.identifier.uri https://doi.org/10.1159/000526245
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/830
dc.description.abstract Introduction: Ghrelin regulates a variety of functions by acting in the brain. The targets of ghrelin in the mouse brain have been mainly mapped using immunolabeling against c-Fos, a transcription factor used as a marker of cellular activation, but such analysis has several limitations. Here, we used positron emission tomography in mice to investigate the brain areas responsive to ghrelin. Methods: We analyzed in male mice the brain areas responsive to systemically injected ghrelin using positron emission tomography imaging of 18F-fluoro-2-deoxyglucose (18F-FDG) uptake, an indicator of metabolic rate. Additionally, we studied if systemic administration of fluorescent ghrelin or native ghrelin displays symmetric accessibility or induction of c-Fos, respectively, in the brain of male mice. Results: Ghrelin increased 18F-FDG uptake in few specific areas of the isocortex, striatum, pallidum, thalamus, and midbrain at 0-10-min posttreatment. At the 10-20 and 20-30 min posttreatment, ghrelin induced mixed changes in 18F-FDG uptake in specific areas of the isocortex, striatum, pallidum, thalamus, and midbrain, as well as in areas of the olfactory areas, hippocampal and retrohippocampal regions, hypothalamus, pons, medulla, and even the cerebellum. Ghrelin-induced changes in 18F-FDG uptake were transient and asymmetric. Systemically administrated fluorescent-ghrelin-labeled midline brain areas known to contain fenestrated capillaries and the hypothalamic arcuate nucleus, where a symmetric labeling was observed. Ghrelin treatment also induced a symmetric increased c-Fos labeling in the arcuate nucleus. Discussion/conclusion: Systemically injected ghrelin transiently and asymmetrically affects the metabolic activity of the brain of male mice in a wide range of areas, in a food intake-independent manner. The neurobiological bases of such asymmetry seem to be independent of the accessibility of ghrelin into the brain. es_ES
dc.language.iso eng es_ES
dc.publisher Karger es_ES
dc.subject Ratones es_ES
dc.subject Mice es_ES
dc.subject Ghrelina es_ES
dc.subject Ghrelin es_ES
dc.title Systemic Ghrelin Treatment Induces Rapid, Transient, and Asymmetric Changes in the Metabolic Activity of the Mouse Brain es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.description.fil Fil: Urrutia, Leandro. Fleni. Departamento de Diagnóstico por Imágenes. Centro de Imágenes Moleculares. Laboratorio De Imágenes Preclínicas; Argentina.
dc.description.fil Fil: De Francesco, Pablo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Fernández, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Uriarte, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Ponce de León, Magdalena. Fleni. Departamento de Diagnóstico por Imágenes. Centro de Imágenes Moleculares. Laboratorio De Imágenes Preclínicas; Argentina.
dc.description.fil Fil: Fehrentz, Jean-Alain. Institut des Biomolécules Max Mousseron-UMR5247; Francia.
dc.description.fil Fil: Falasco, Germán. Fleni. Departamento de Diagnóstico por Imágenes. Centro de Imágenes Moleculares. Laboratorio De Imágenes Preclínicas; Argentina.
dc.description.fil Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Uppsala; Suecia.
dc.relation.ispartofCOUNTRY Suiza
dc.relation.ispartofCITY Basilea
dc.relation.ispartofISSN 1423-0194
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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