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Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT

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dc.contributor.author Aban, Cyntia
dc.contributor.author Lombardi, Antonella
dc.contributor.author Neiman, Gabriel
dc.contributor.author Biani, María Celeste
dc.contributor.author La Greca, Alejandro
dc.contributor.author Waisman, Ariel
dc.contributor.author Moro, Lucía Natalia
dc.contributor.author Sevlever, Gustavo Emilio
dc.contributor.author Miriuka, Santiago Gabriel
dc.contributor.author Luzzani, Carlos Daniel
dc.date.accessioned 2021-01-26T14:55:37Z
dc.date.available 2021-01-26T14:55:37Z
dc.date.issued 2021-01-21
dc.identifier.citation Aban C, Lombardi A, Neiman G, Biani MC, La Greca A, Waisman A, Moro LN, Sevlever G, Miriuka S, Luzzani CD. Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT. Sci Rep. 2021 Jan 21;11(1):2048. doi: 10.1038/s41598-021-81735-1. en_US
dc.identifier.uri https://doi.org/10.1038/s41598-021-81735-1
dc.identifier.uri https://repositorio.fleni.org.ar/handle/123456789/342
dc.description.abstract Epithelial to mesenchymal transition (EMT) is a critical cellular process that has been well characterized during embryonic development and cancer metastasis and it also is implicated in several physiological and pathological events including embryonic stem cell differentiation. During early stages of differentiation, human embryonic stem cells pass through EMT where deeper morphological, molecular and biochemical changes occur. Though initially considered as a decision between two states, EMT process is now regarded as a fluid transition where cells exist on a spectrum of intermediate states. In this work, using a CRISPR interference system in human embryonic stem cells, we describe a molecular characterization of the effects of downregulation of E-cadherin, one of the main initiation events of EMT, as a unique start signal. Our results suggest that the decrease and delocalization of E-cadherin causes an incomplete EMT where cells retain their undifferentiated state while expressing several characteristics of a mesenchymal-like phenotype. Namely, we found that E-cadherin downregulation induces SNAI1 and SNAI2 upregulation, promotes MALAT1 and LINC-ROR downregulation, modulates the expression of tight junction occludin 1 and gap junction connexin 43, increases human embryonic stem cells migratory capacity and delocalize β-catenin. Altogether, we believe our results provide a useful tool to model the molecular events of an unstable intermediate state and further identify multiple layers of molecular changes that occur during partial EMT. en_US
dc.language.iso eng en_US
dc.publisher Nature Publishing Group en_US
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Cadherins en_US
dc.subject Cadherinas en_US
dc.subject Pluripotent Stem Cells en_US
dc.subject Células Madre Pluripotentes en_US
dc.subject Epithelial-Mesenchymal Transition en_US
dc.subject Transición Epitelial-Mesenquimal en_US
dc.title Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT en_US
dc.type info:eu-repo/semantics/publishedVersion
dc.type info:eu-repo/semantics/article en_US
dc.description.fil Fil: Aban, Cyntia. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Lombardi, Antonella. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Neiman, Gabriel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Biani, María Celeste. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: La Greca, Alejandro. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Waisman, Ariel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Moro, Lucía Natalia. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Sevlever, Gustavo Emilio. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Miriuka, Santiago Gabriel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Luzzani, Carlos Daniel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.relation.ispartofVOLUME 11
dc.relation.ispartofNUMBER 1
dc.relation.ispartofCOUNTRY Reino Unido
dc.relation.ispartofCITY Londres
dc.relation.ispartofISSN 2045-2322
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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