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Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism

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dc.contributor.author Isaja, Luciana
dc.contributor.author Mucci, Sofía
dc.contributor.author Vera, Jonathan
dc.contributor.author Rodríguez Varela, María Soledad
dc.contributor.author Marazita, Mariela Claudia
dc.contributor.author Morris-Hanon, Olivia
dc.contributor.author Videla Richardson, Guillermo Agustín
dc.contributor.author Sevlever, Gustavo Emilio
dc.contributor.author Scassa, María Élida
dc.contributor.author Romorini, Leonardo
dc.date.accessioned 2021-04-22T12:31:36Z
dc.date.available 2021-04-22T12:31:36Z
dc.date.issued 2020-10-26
dc.identifier.citation Isaja, L., Mucci, S., Vera, J., Rodríguez-Varela, M.S., Marazita, M., Morris-Hanon, O., Videla-Richardson, G.A., Sevlever, G.E., Scassa, M.E., Romorini, L., 2020. Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism. Sci Rep 10, 20653. https://doi.org/10.1038/s41598-020-77792-7 es_ES
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/427
dc.identifier.uri https://www.nature.com/articles/s41598-020-77792-7
dc.description.abstract Human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) are self-renewing human pluripotent stem cells (hPSCs) that can differentiate to a wide range of specialized cells. Notably, hPSCs enhance their undifferentiated state and self-renewal properties in hypoxia (5% O2). Although thoroughly analyzed, hypoxia implication in hPSCs death is not fully determined. In order to evaluate the effect of chemically mimicked hypoxia on hPSCs cell survival, we analyzed changes in cell viability and several aspects of apoptosis triggered by CoCl2 and dimethyloxalylglycine (DMOG). Mitochondrial function assays revealed a decrease in cell viability at 24 h post-treatments. Moreover, we detected chromatin condensation, DNA fragmentation and CASPASE-9 and 3 cleavages. In this context, we observed that P53, BNIP-3, and NOXA protein expression levels were significantly up-regulated at different time points upon chemical hypoxia induction. However, only siRNA-mediated downregulation of NOXA but not HIF-1α, HIF-2α, BNIP-3, and P53 did significantly affect the extent of cell death triggered by CoCl2 and DMOG in hPSCs. In conclusion, chemically mimicked hypoxia induces hPSCs cell death by a NOXA-mediated HIF-1α and HIF-2α independent mechanism. es_ES
dc.language.iso eng es_ES
dc.publisher Nature Publishing Group es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Apoptosis es_ES
dc.subject Hypoxia es_ES
dc.subject Hipoxia es_ES
dc.subject Pluripotent Stem Cells es_ES
dc.subject Células Madre Pluripotentes es_ES
dc.title Chemical hypoxia induces apoptosis of human pluripotent stem cells by a NOXA-mediated HIF-1α and HIF-2α independent mechanism es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Sevlever, Gustavo Emilio. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Isaja, Luciana. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Mucci, Sofía. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Vera, Jonathan. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Rodríguez Varela, María Soledad. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Marazita, Mariela Claudia. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Morris-Hanon, Olivia. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Videla Richardson, Guillermo Agustín. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Scassa, María Élida. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Romorini, Leonardo. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.relation.ispartofVOLUME 10
dc.relation.ispartofNUMBER 1
dc.relation.ispartofPAGINATION 20653
dc.relation.ispartofCOUNTRY Inglaterra
dc.relation.ispartofCITY Londres
dc.relation.ispartofTITLE Scientific reports
dc.relation.ispartofISSN 2045-2322
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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