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Generation of myostatin edited horse embryos using CRISPR/Cas9 technology and somatic cell nuclear transfer

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dc.contributor.author Moro, Lucía Natalia
dc.contributor.author Viale, Diego Luis
dc.contributor.author Bastón, Juan Ignacio
dc.contributor.author Arnold, Victoria
dc.contributor.author Suva, Mariana
dc.contributor.author Wiedenmann, Elisabet
dc.contributor.author Olguín, Martín
dc.contributor.author Miriuka, Santiago Gabriel
dc.contributor.author Vichera, Gabriel
dc.date.accessioned 2021-04-29T15:17:49Z
dc.date.available 2021-04-29T15:17:49Z
dc.date.issued 2020-08-24
dc.identifier.citation Moro, L.N., Viale, D.L., Bastón, J.I., Arnold, V., Suvá, M., Wiedenmann, E., Olguín, M., Miriuka, S., Vichera, G., 2020. Generation of myostatin edited horse embryos using CRISPR/Cas9 technology and somatic cell nuclear transfer. Sci Rep 10, 15587. https://doi.org/10.1038/s41598-020-72040-4 es_ES
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/444
dc.identifier.uri https://www.nature.com/articles/s41598-020-72040-4.pdf
dc.description.abstract The application of new technologies for gene editing in horses may allow the generation of improved sportive individuals. Here, we aimed to knock out the myostatin gene (MSTN), a negative regulator of muscle mass development, using CRISPR/Cas9 and to generate edited embryos for the first time in horses. We nucleofected horse fetal fibroblasts with 1, 2 or 5 µg of 2 different gRNA/Cas9 plasmids targeting the first exon of MSTN. We observed that increasing plasmid concentrations improved mutation efficiency. The average efficiency was 63.6% for gRNA1 (14/22 edited clonal cell lines) and 96.2% for gRNA2 (25/26 edited clonal cell lines). Three clonal cell lines were chosen for embryo generation by somatic cell nuclear transfer: one with a monoallelic edition, one with biallelic heterozygous editions and one with a biallelic homozygous edition, which rendered edited blastocysts in each case. Both MSTN editions and off-targets were analyzed in the embryos. In conclusion, CRISPR/Cas9 proved an efficient method to edit the horse genome in a dose dependent manner with high specificity. Adapting this technology sport advantageous alleles could be generated, and a precision breeding program could be developed. es_ES
dc.language.iso eng es_ES
dc.publisher Nature Publishing Group es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject CRISPR-Cas Systems es_ES
dc.subject Sistemas CRISPR-Cas es_ES
dc.subject Myostatin es_ES
dc.subject Miostatina es_ES
dc.title Generation of myostatin edited horse embryos using CRISPR/Cas9 technology and somatic cell nuclear transfer es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Miriuka, Santiago Gabriel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina.
dc.description.fil Fil: Moro, Lucía Natalia. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Viale, Diego Luis. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina. Laboratorio de Neurología y Citogenética Molecular, CESyMA; Argentina.
dc.description.fil Fil: Bastón, Juan Ignacio. KHEIRON BIOTECH; Argentina.
dc.description.fil Fil: Arnold, Victoria. KHEIRON BIOTECH; Argentina.
dc.description.fil Fil: Suva, Mariana. KHEIRON BIOTECH; Argentina.
dc.description.fil Fil: Wiedenmann, Elisabet. KHEIRON BIOTECH; Argentina.
dc.description.fil Fil: Olguín, Martín. KHEIRON BIOTECH; Argentina.
dc.description.fil Fil: Vichera, Gabriel. KHEIRON BIOTECH; Argentina.
dc.relation.ispartofVOLUME 10
dc.relation.ispartofNUMBER 1
dc.relation.ispartofPAGINATION 15587
dc.relation.ispartofCOUNTRY Inglaterra
dc.relation.ispartofCITY Londres
dc.relation.ispartofTITLE Scientific reports.
dc.relation.ispartofISSN 2045-2322
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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