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Wound Healing by Allogeneic Transplantation of Specific Subpopulation From Human Umbilical Cord Mesenchymal Stem Cells

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dc.contributor.author Palma, María Belén
dc.contributor.author Luzzani, Carlos Daniel
dc.contributor.author Andrini, Laura B.
dc.contributor.author Riccillo, Fernando
dc.contributor.author Buero, Guillermo
dc.contributor.author Pelinski, Pablo
dc.contributor.author Inda, Ana María
dc.contributor.author Errecalde, Ana Lía
dc.contributor.author Miriuka, Santiago Gabriel
dc.contributor.author Carosella, Edgardo D.
dc.contributor.author Garcia, Marcela N.
dc.date.accessioned 2021-06-15T13:36:12Z
dc.date.available 2021-06-15T13:36:12Z
dc.date.issued 2021-05-11
dc.identifier.citation Palma MB, Luzzani CD, Andrini LB, Riccillo F, Buero G, Pelinski P, Inda AM, Errecalde AL, Miriuka S, Carosella ED, Garcia MN. Wound Healing by Allogeneic Transplantation of Specific Subpopulation From Human Umbilical Cord Mesenchymal Stem Cells. Cell Transplant. 2021 Jan-Dec;30:963689721993774. doi: 10.1177/0963689721993774 es_ES
dc.identifier.uri https://doi.org/10.1177/0963689721993774
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/492
dc.description.abstract In normal physiological conditions, restoration of a functional epidermal barrier is highly efficient; nevertheless, when it fails, one of the main consequences is a chronic ulcerative skin defect, one of the most frequently recognized complications of diabetes. Most of these chronic venous ulcers do not heal with conventional treatment, leading to the appearance of infections and complications in the patient. Treatments based on the use of autologous mesenchymal stem cells (MSC) have been successful; however, its implementation entails complications. The umbilical cord offers an unlimited source of adult MSC (ucMSC) from the Wharton’s jelly tissue with the same relevant features for clinical applicability and avoiding difficulties. It has recently been characterized by one specific subpopulation derived from ucMSC, the differentiated mesenchymal cells (DMCs). This subpopulation expresses the human leukocyte antigen-G (HLA-G) molecule, a strong immunosuppressive checkpoint, and vascular endothelial growth factor (VEGF), the most potent angiogenic factor. Considering the importance of developing a more effective therapy for wound treatment, especially ulcerative skin lesions, we analyzed DMC safety, efficacy, and therapeutic potential. By immunohistochemistry, umbilical cords HLA-G and VEGF positive were selected. Flow cytometry revealed that 90% of the DMC subpopulation are HLA-G+, CD44+, CD73+, CD29+, CD105+, CD90+, and HLA-DR−. Reverse transcription-polymerase chain reaction revealed the expression of HLA-G in all of DMC subpopulations. Upon co-culture with the DMC, peripheral blood mononuclear cell proliferation was inhibited by 50%. In a xenograft transplantation assay, DMC improved wound healing with no signs of rejection of the transplanted cells in immunocompetent mice. This study confirms that HLA-G allows allogeneic cell transplantation, and VEGF is fundamental for the restoration of the failure in blood supply. DMC population has positive effects on wound healing by promoting local angiogenesis in skin lesions. DMC could play a very important role in regenerative medicine and could be a novel allogeneic cell-therapeutic tool for wound healing. es_ES
dc.language.iso eng es_ES
dc.publisher Sage es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Wound Healing es_ES
dc.subject Cicatrización de Heridas es_ES
dc.subject Transplantation, Homologous es_ES
dc.subject Trasplante Homólogo es_ES
dc.subject Varicose Ulcer es_ES
dc.subject Úlcera Varicosa es_ES
dc.title Wound Healing by Allogeneic Transplantation of Specific Subpopulation From Human Umbilical Cord Mesenchymal Stem Cells es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Palma, María Belén. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Cátedra de Citología, Histología y Embriología; Argentina. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Luzzani, Carlos Daniel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Andrini, Laura B. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Cátedra de Citología, Histología y Embriología; Argentina.
dc.description.fil Fil: Riccillo, Fernando. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Cátedra de Citología, Histología y Embriología; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina.
dc.description.fil Fil: Buero, Guillermo. Sanatorio Mater Dei; Argentina.
dc.description.fil Fil: Pelinski, Pablo. Hospital Español; Argentina.
dc.description.fil Fil: Inda, Ana María. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Cátedra de Citología, Histología y Embriología; Argentina. Comisión de Investigaciones Científicas; Argentina.
dc.description.fil Fil: Errecalde, Ana Lía. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Cátedra de Citología, Histología y Embriología; Argentina.
dc.description.fil Fil: Miriuka, Santiago. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Cátedra de Citología, Histología y Embriología; Argentina. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Carosella, Edgardo D. Saint-Louis Hospital; Francia.
dc.description.fil Fil: Garcia, Marcela N. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Cátedra de Citología, Histología y Embriología; Argentina.
dc.relation.ispartofCOUNTRY Estados Unidos
dc.relation.ispartofCITY Thousand Oaks
dc.relation.ispartofTITLE Cell transplantation
dc.relation.ispartofISSN 1555-3892
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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