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dc.contributor.author | Mucci, Sofía | |
dc.contributor.author | Rodríguez Varela, María Soledad | |
dc.contributor.author | Isaja, Luciana | |
dc.contributor.author | Ferriol Laffouillere, Sofía Luján | |
dc.contributor.author | Sevlever, Gustavo Emilio | |
dc.contributor.author | Scassa, María Élida | |
dc.contributor.author | Romorini, Leonardo | |
dc.date.accessioned | 2022-07-25T15:57:03Z | |
dc.date.available | 2022-07-25T15:57:03Z | |
dc.date.issued | 2022-06-18 | |
dc.identifier.citation | Mucci S, Rodriguez-Varela MS, Isaja L, Ferriol-Laffouillere SL, Sevlever GE, Scassa ME, Romorini L. Protocol for morphometric analysis of neurons derived from human pluripotent stem cells. STAR Protoc. 2022 Jun 18;3(3):101487. doi: 10.1016/j.xpro.2022.101487. | es_ES |
dc.identifier.uri | https://repositorio.fleni.org.ar/xmlui/handle/123456789/636 | |
dc.identifier.uri | https://doi.org/10.1016/j.xpro.2022.101487 | |
dc.description.abstract | The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Cell Press | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.subject | Molecular Biology | es_ES |
dc.subject | Biología Molecular | es_ES |
dc.subject | Stem Cells | es_ES |
dc.subject | Células Madre | es_ES |
dc.subject | Cell Biology | es_ES |
dc.subject | Biología Celular | es_ES |
dc.title | Protocol for morphometric analysis of neurons derived from human pluripotent stem cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.description.fil | Fil: Mucci, Sofía. Fleni. Laboratorio de Investigación Aplicada en Neurociencias; Argentina. | |
dc.description.fil | Fil: Rodríguez Varela, María Soledad. Fleni. Laboratorio de Investigación Aplicada en Neurociencias; Argentina. | |
dc.description.fil | Fil: Isaja, Luciana. Fleni. Laboratorio de Investigación Aplicada en Neurociencias; Argentina. | |
dc.description.fil | Fil: Ferriol-Laffouillere, Sofía Luján. Fleni. Laboratorio de Investigación Aplicada en Neurociencias; Argentina. | |
dc.description.fil | Fil: Sevlever, Gustavo Emilio. Fleni. Laboratorio de Investigación Aplicada en Neurociencias; Argentina. Fleni. Departamento de Neuropatología y Biología Molecular. Laboratorio de Enfermedades Neurodegenerativas; Argentina. | |
dc.description.fil | Fil: Scassa, María Élida. Fleni. Laboratorio de Investigación Aplicada en Neurociencias; Argentina. | |
dc.description.fil | Fil: Romorini, Leonardo. Fleni. Laboratorio de Investigación Aplicada en Neurociencias; Argentina. | |
dc.relation.ispartofVOLUME | 3 | |
dc.relation.ispartofNUMBER | 3 | |
dc.relation.ispartofPAGINATION | 101487 | |
dc.relation.ispartofCOUNTRY | Estados Unidos | |
dc.relation.ispartofCITY | Cambridge | |
dc.relation.ispartofTITLE | STAR protocols | |
dc.relation.ispartofISSN | 2666-1667 | |
dc.type.snrd | info:ar-repo/semantics/artículo | es_ES |