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Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system

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dc.contributor.author Pérez-Sosa, Camilo
dc.contributor.author Sanluis-Verdes, Anahí
dc.contributor.author Waisman, Ariel
dc.contributor.author Lombardi, Antonella
dc.contributor.author Rosero, Gustavo
dc.contributor.author La Greca, Alejandro
dc.contributor.author Bhansali, Shekhar
dc.contributor.author Bourguignon, Natalia
dc.contributor.author Luzzani, Carlos Daniel
dc.contributor.author Pérez, Maximiliano S.
dc.contributor.author Miriuka, Santiago Gabriel
dc.contributor.author Lerner, Betiana
dc.date.accessioned 2022-10-24T12:25:30Z
dc.date.available 2022-10-24T12:25:30Z
dc.date.issued 2022-01-12
dc.identifier.citation Pérez-Sosa C, Sanluis-Verdes A, Waisman A, Lombardi A, Rosero G, Greca A, Bhansali S, Bourguignon N, Luzzani CD, Pérez MS, Miriuka S, Lerner B. Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system. R Soc Open Sci. 2022 Jan 12;9(1):211510. doi: 10.1098/rsos.211510. es_ES
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/704
dc.identifier.uri https://doi.org/10.1098/rsos.211510
dc.description.abstract Microfluidic tools have recently made possible many advances in biological and biomedical research. Research in fields such as physics, engineering, chemistry and biology have combined to produce innovation in microfluidics which has positively impacted diverse areas such as nucleotide sequencing, functional genomics, single-cell studies, single molecules assays and biomedical diagnostics. Among these areas, regenerative medicine and stem cells have benefited from microfluidics since these tools have had a profound impact on their applications. In this study, we present a high-performance droplet-based system for transfecting individual human-induced pluripotent stem cells. We will demonstrate that this system has great efficiency in single cells and captured droplets, like other microfluidic methods but with lower cost. Moreover, this microfluidic approach can be associated with the PiggyBac transposase-based system to increase its transfection efficiency. Our results provide a starting point for subsequent applications in more complex transfection systems, single-cell differentiation interactions, cell subpopulations and cell therapy, among other potential applications. es_ES
dc.language.iso eng es_ES
dc.publisher Royal Society Publishing es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Transfección es_ES
dc.subject Transfection es_ES
dc.subject Microfluídica es_ES
dc.subject Microfluidics es_ES
dc.title Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Pérez-Sosa, Camilo. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina.
dc.description.fil Fil: Sanluis-Verdes, Anahí. Universidad Tecnológica Nacional; Argentina.
dc.description.fil Fil: Waisman, Ariel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Lombardi, Antonella. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Rosero, Gustavo. Universidad Tecnológica Nacional; Argentina.
dc.description.fil Fil: La Greca, Alejandro. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Bhansali, Shekhar. Florida International University. Department of Electrical and Computer Engineering; Estados Unidos.
dc.description.fil Fil: Bourguignon, Natalia. Universidad Tecnológica Nacional; Argentina. Florida International University. Department of Electrical and Computer Engineering; Estados Unidos.
dc.description.fil Fil: Luzzani, Carlos Daniel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Pérez, Maximiliano S. Florida International University. Department of Electrical and Computer Engineering; Estados Unidos. Universidad de Buenos Aires. Institute of Biomedical Engineering; Argentina.
dc.description.fil Fil: Miriuka, Santiago Gabriel. Fleni. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Florida International University. Department of Electrical and Computer Engineering; Estados Unidos.
dc.relation.ispartofVOLUME 9
dc.relation.ispartofNUMBER 1
dc.relation.ispartofPAGINATION 211510
dc.relation.ispartofCOUNTRY Inglaterra
dc.relation.ispartofCITY Londres
dc.relation.ispartofTITLE Royal Society open science
dc.relation.ispartofISSN 2054-5703
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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