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Iron metabolism in oligodendrocytes and astrocytes: friend or foe?

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dc.contributor.author Pasquini, Juana María
dc.contributor.author Rosato-Siri, María Victoria
dc.contributor.author Martino Adami, Pamela V.
dc.contributor.author Guitart, María Eugenia
dc.contributor.author Marcora, María Silvina
dc.contributor.author Morelli, Laura
dc.contributor.author Correale, Jorge
dc.date.accessioned 2023-01-11T14:44:08Z
dc.date.available 2023-01-11T14:44:08Z
dc.date.issued 2021-11
dc.identifier.citation JM Pasquini, MV Rosato Siri, PV Martino Adami, ME Guitart, MS Marcora, L Morelli, J Correale. Iron metabolism in oligodendrocytes and astrocytes: friend or foe? Annual Meeting of Bioscience Societies November 17-20 2021. MEDICINA (Buenos Aires) 2021; 81 (Supl. III) es_ES
dc.identifier.uri https://medicinabuenosaires.com/revistas/vol81-21/s3/Mv81s3.pdf
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/771
dc.description.abstract Recent reports show that astrocytes (AST) are able to create a permissive environment for remyelination through their action on oligodendrocyte (OLG) precursor migration, proliferation, and differentiation. When disrupted, iron homeostasis negatively impacts OLG differentiation and impairs myelination. We demonstrate that iron deficiency (ID) affects not only OLG maturation but also AST. Using gestational iron deprivation, we studied OLG requirements for their progression to a mature myelinating state and energy metabolism in primary cultures of OLG and AST from newly born control and ID pups. In particular, oxygen consumption and extracellular acidification rates were measured using a Seahorse extracellular flux analyzer. Both ID AST and OLG exhibited decreased spare respiratory capacity, which indicates that maternal ID effectively induces mitochondrial dysfunction. Absence of glycogen granules was observed in ID AST and an increase in ROS production was detected in ID OLG. Mitochondrial fission was increased in ID AST, while fusion was prevalent in ID OLG. Electron microscopy also showed abnormal cristae in ID mitochondria in OLG as well as in AST. These findings further prove that the regulation of cell metabolism may impact cell fate decisions and maturation. An additional model of ID was developed by knocking down the divalent metal transporter 1 (DMT1), a multi-metal transporter with a primary role in iron transport and present in AST and OLG. OLG maturation was compromised in primary OPC cultures treated with conditioned medium from DMT1-silenced AST, which suggests that molecules secreted by AST may be affected. es_ES
dc.language.iso eng es_ES
dc.publisher Reunión de Sociedades de Biociencias es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Oligodendroglia es_ES
dc.subject Astrocytes es_ES
dc.title Iron metabolism in oligodendrocytes and astrocytes: friend or foe? es_ES
dc.type Presentation es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Pasquini, Juana María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina.
dc.description.fil Fil: Rosato-Siri, María Victoria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina.
dc.description.fil Fil: Martino Adami, P.V. Instituto de Investigaciones Bioquímicas de Buenos Aires. Laboratorio de Envejecimiento y Neurodegeneración; Argentina.
dc.description.fil Fil: Guitart, María Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina.
dc.description.fil Fil: Marcora, Maria Silvina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina.
dc.description.fil Fil: Morelli, Laura. Instituto de Investigaciones Bioquímicas de Buenos Aires. Laboratorio de Envejecimiento y Neurodegeneración; Argentina.
dc.description.fil Fil: Correale, Jorge. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina.
dc.relation.ispartofVOLUME 81
dc.relation.ispartofNUMBER Suplemento 3
dc.relation.ispartofPAGINATION 20
dc.relation.ispartofCOUNTRY Argentina
dc.relation.ispartofCITY Buenos Aires
dc.relation.ispartofTITLE Medicina
dc.relation.ispartofISSN 1669-9106
dc.type.snrd Presentation es_ES


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