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dc.contributor.author | Garcia Fallit, Matías | |
dc.contributor.author | Pidre, Matías L. | |
dc.contributor.author | Asad, Antonela S. | |
dc.contributor.author | Peña Agudelo, Jorge A. | |
dc.contributor.author | Vera, Mariana Belén | |
dc.contributor.author | Nicola Candia, Alejandro J. | |
dc.contributor.author | Sagripanti, Sofia B. | |
dc.contributor.author | Pérez Kuper, Melanie | |
dc.contributor.author | Amorós Morales, Leslie C. | |
dc.contributor.author | Marchesini, Abril | |
dc.contributor.author | Gonzalez, Nazareno | |
dc.contributor.author | Caruso, Carla M. | |
dc.contributor.author | Romanowski, Víctor | |
dc.contributor.author | Seilicovich, Adriana | |
dc.contributor.author | Videla Richardson, Guillermo Agustín | |
dc.contributor.author | Zanetti, Flavia A. | |
dc.contributor.author | Candolfi, Marianela | |
dc.date.accessioned | 2023-08-09T16:39:05Z | |
dc.date.available | 2023-08-09T16:39:05Z | |
dc.date.issued | 2023-02-22 | |
dc.identifier.citation | Garcia Fallit, M., Pidre, M.L., Asad, A.S., Agudelo, J.A.P., Vera, M.B., Candia, A.J.N., Sagripanti, S.B., Kuper, M.P., Morales, L.C.A., Marchesini, A., Gonzalez, N., Caruso, C.M., Romanowski, V., Seilicovich, A., Videla-Richardson, G.A., Zanetti, F.A., Candolfi, M., 2023. Evaluation of Baculoviruses as Gene Therapy Vectors for Brain Cancer. Viruses 15, 608. https://doi.org/10.3390/v15030608 | es_ES |
dc.identifier.uri | https://doi.org/10.3390/v15030608 | |
dc.identifier.uri | https://repositorio.fleni.org.ar/xmlui/handle/123456789/803 | |
dc.description.abstract | We aimed to assess the potential of baculoviral vectors (BV) for brain cancer gene therapy. We compared them with adenoviral vectors (AdV), which are used in neuro-oncology, but for which there is pre-existing immunity. We constructed BVs and AdVs encoding fluorescent reporter proteins and evaluated their transduction efficiency in glioma cells and astrocytes. Naïve and glioma-bearing mice were intracranially injected with BVs to assess transduction and neuropathology. Transgene expression was also assessed in the brain of BV-preimmunized mice. While the expression of BVs was weaker than AdVs in murine and human glioma cell lines, BV-mediated transgene expression in patient-derived glioma cells was similar to AdV-mediated transduction and showed strong correlation with clathrin expression, a protein that interacts with the baculovirus glycoprotein GP64, mediating BV endocytosis. BVs efficiently transduced normal and neoplastic astrocytes in vivo, without apparent neurotoxicity. BV-mediated transgene expression was stable for at least 21 days in the brain of naïve mice, but it was significantly reduced after 7 days in mice systemically preimmunized with BVs. Our findings indicate that BVs efficiently transduce glioma cells and astrocytes without apparent neurotoxicity. Since humans do not present pre-existing immunity against BVs, these vectors may constitute a valuable tool for the delivery of therapeutic genes into the brain. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Adenoviridae | es_ES |
dc.subject | Baculoviridae | es_ES |
dc.subject | Brain Neoplasms | es_ES |
dc.subject | Neoplasias Encefálicas | es_ES |
dc.subject | Genetic Therapy | es_ES |
dc.subject | Terapia Genética | es_ES |
dc.subject | Glioma | es_ES |
dc.title | Evaluation of Baculoviruses as Gene Therapy Vectors for Brain Cancer | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.description.fil | Fil: Videla Richardson, Guillermo Agustín. Fleni. Instituto de Neurociencias FLENI-CONICET. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.description.fil | Fil: Vera, Mariana Belén. Fleni. Instituto de Neurociencias FLENI-CONICET. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | |
dc.relation.ispartofVOLUME | 15 | |
dc.relation.ispartofNUMBER | 3 | |
dc.relation.ispartofPAGINATION | 608 | |
dc.relation.ispartofCOUNTRY | Suiza | |
dc.relation.ispartofCITY | Basilea | |
dc.relation.ispartofTITLE | Viruses | |
dc.relation.ispartofISSN | 1999-4915 | |
dc.type.snrd | info:ar-repo/semantics/artículo | es_ES |