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Evaluation of Baculoviruses as Gene Therapy Vectors for Brain Cancer

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dc.contributor.author Garcia Fallit, Matías
dc.contributor.author Pidre, Matías L.
dc.contributor.author Asad, Antonela S.
dc.contributor.author Peña Agudelo, Jorge A.
dc.contributor.author Vera, Mariana Belén
dc.contributor.author Nicola Candia, Alejandro J.
dc.contributor.author Sagripanti, Sofia B.
dc.contributor.author Pérez Kuper, Melanie
dc.contributor.author Amorós Morales, Leslie C.
dc.contributor.author Marchesini, Abril
dc.contributor.author Gonzalez, Nazareno
dc.contributor.author Caruso, Carla M.
dc.contributor.author Romanowski, Víctor
dc.contributor.author Seilicovich, Adriana
dc.contributor.author Videla Richardson, Guillermo Agustín
dc.contributor.author Zanetti, Flavia A.
dc.contributor.author Candolfi, Marianela
dc.date.accessioned 2023-08-09T16:39:05Z
dc.date.available 2023-08-09T16:39:05Z
dc.date.issued 2023-02-22
dc.identifier.citation Garcia Fallit, M., Pidre, M.L., Asad, A.S., Agudelo, J.A.P., Vera, M.B., Candia, A.J.N., Sagripanti, S.B., Kuper, M.P., Morales, L.C.A., Marchesini, A., Gonzalez, N., Caruso, C.M., Romanowski, V., Seilicovich, A., Videla-Richardson, G.A., Zanetti, F.A., Candolfi, M., 2023. Evaluation of Baculoviruses as Gene Therapy Vectors for Brain Cancer. Viruses 15, 608. https://doi.org/10.3390/v15030608 es_ES
dc.identifier.uri https://doi.org/10.3390/v15030608
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/803
dc.description.abstract We aimed to assess the potential of baculoviral vectors (BV) for brain cancer gene therapy. We compared them with adenoviral vectors (AdV), which are used in neuro-oncology, but for which there is pre-existing immunity. We constructed BVs and AdVs encoding fluorescent reporter proteins and evaluated their transduction efficiency in glioma cells and astrocytes. Naïve and glioma-bearing mice were intracranially injected with BVs to assess transduction and neuropathology. Transgene expression was also assessed in the brain of BV-preimmunized mice. While the expression of BVs was weaker than AdVs in murine and human glioma cell lines, BV-mediated transgene expression in patient-derived glioma cells was similar to AdV-mediated transduction and showed strong correlation with clathrin expression, a protein that interacts with the baculovirus glycoprotein GP64, mediating BV endocytosis. BVs efficiently transduced normal and neoplastic astrocytes in vivo, without apparent neurotoxicity. BV-mediated transgene expression was stable for at least 21 days in the brain of naïve mice, but it was significantly reduced after 7 days in mice systemically preimmunized with BVs. Our findings indicate that BVs efficiently transduce glioma cells and astrocytes without apparent neurotoxicity. Since humans do not present pre-existing immunity against BVs, these vectors may constitute a valuable tool for the delivery of therapeutic genes into the brain. es_ES
dc.language.iso eng es_ES
dc.publisher MDPI es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.subject Adenoviridae es_ES
dc.subject Baculoviridae es_ES
dc.subject Brain Neoplasms es_ES
dc.subject Neoplasias Encefálicas es_ES
dc.subject Genetic Therapy es_ES
dc.subject Terapia Genética es_ES
dc.subject Glioma es_ES
dc.title Evaluation of Baculoviruses as Gene Therapy Vectors for Brain Cancer es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Videla Richardson, Guillermo Agustín. Fleni. Instituto de Neurociencias FLENI-CONICET. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.description.fil Fil: Vera, Mariana Belén. Fleni. Instituto de Neurociencias FLENI-CONICET. Laboratorio de Investigación Aplicada a las Neurociencias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
dc.relation.ispartofVOLUME 15
dc.relation.ispartofNUMBER 3
dc.relation.ispartofPAGINATION 608
dc.relation.ispartofCOUNTRY Suiza
dc.relation.ispartofCITY Basilea
dc.relation.ispartofTITLE Viruses
dc.relation.ispartofISSN 1999-4915
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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