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Remyelinating effect driven by transferrin-loaded extracellular vesicles

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dc.contributor.author Mattera, Vanesa S.
dc.contributor.author Occhiuzzi, Federico
dc.contributor.author Correale, Jorge
dc.contributor.author Pasquini, Juana María
dc.date.accessioned 2024-03-22T13:49:43Z
dc.date.available 2024-03-22T13:49:43Z
dc.date.issued 2023-10-20
dc.identifier.citation Mattera V, Occhiuzzi F, Correale J, Pasquini JM. Remyelinating effect driven by transferrin-loaded extracellular vesicles. Glia. febrero de 2024;72(2):338-61. es_ES
dc.identifier.uri https://doi.org/10.1002/glia.24478
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/1046
dc.description.abstract Extracellular vesicles (EVs) are involved in diverse cellular functions, playing a significant role in cell-to-cell communication in both physiological conditions and pathological scenarios. Therefore, EVs represent a promising therapeutic strategy. Oligodendrocytes (OLs) are myelinating glial cells developed from oligodendrocyte progenitor cells (OPCs) and damaged in chronic demyelinating diseases such as multiple sclerosis (MS). Glycoprotein transferrin (Tf) plays a critical role in iron homeostasis and has pro-differentiating effects on OLs in vivo and in vitro. In the current work, we evaluated the use of EVs as transporters of Tf to the central nervous system (CNS) through the intranasal (IN) route. For the in vitro mechanistic studies, we used rat plasma EVs. Our results show that EVTf enter OPCs through clathrin-caveolae and cholesterol-rich lipid raft endocytic pathways, releasing the cargo and exerting a pro-maturation effect on OPCs. These effects were also observed in vivo using the animal model of demyelination induced by cuprizone (CPZ). In this model, IN administered Tf-loaded EVs isolated from mouse plasma reached the brain parenchyma, internalizing into OPCs, promoting their differentiation, and accelerating remyelination. Furthermore, in vivo experiments demonstrated that EVs protected the Tf cargo and significantly reduced the amount of Tf required to induce remyelination as compared to soluble Tf. Collectively, these findings unveil EVs as functional nanocarriers of Tf to induce remyelination. es_ES
dc.language.iso eng es_ES
dc.publisher Wiley es_ES
dc.subject Cell Differentiation es_ES
dc.subject Diferenciación Celular es_ES
dc.subject Cuprizone es_ES
dc.subject Cuprizona es_ES
dc.subject Demyelinating Diseases es_ES
dc.subject Enfermedades Desmielinizantes es_ES
dc.subject Extracellular Vesicles es_ES
dc.subject Vesículas Extracelulares es_ES
dc.subject Myelin Sheath es_ES
dc.subject Vaina de Mielina es_ES
dc.subject Oligodendroglia es_ES
dc.subject Transferrin es_ES
dc.subject Transferrina es_ES
dc.title Remyelinating effect driven by transferrin-loaded extracellular vesicles es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.description.fil Fil: Correale, Jorge. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina.
dc.relation.ispartofVOLUME 72
dc.relation.ispartofNUMBER 2
dc.relation.ispartofPAGINATION 338-361
dc.relation.ispartofCOUNTRY Estados Unidos
dc.relation.ispartofCITY Nueva York
dc.relation.ispartofTITLE Glia
dc.relation.ispartofISSN 1098-1136
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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