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Mucosal associated invariant T cell features and TCR repertoire characteristics during the course of Multiple Sclerosis

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dc.contributor.author Carnero Contentti, Edgar
dc.contributor.author Farez, Mauricio Franco
dc.contributor.author Correale, Jorge
dc.date.accessioned 2019-11-15T14:22:02Z
dc.date.available 2019-11-15T14:22:02Z
dc.date.issued 2019-10-31
dc.identifier.citation Carnero Contentti E, Farez MF, Correale J. Mucosal associated invariant T cell features and TCR repertoire characteristics during the course of Multiple Sclerosis. Front Immunol. 2019;10. doi:10.3389/fimmu.2019.02690 en_US
dc.identifier.uri https://repositorio.fleni.org.ar/handle/123456789/104
dc.description.abstract Objective: To investigate the frequency, phenotype, function and longitudinal repertoire of Mucosal-associated invariant T (MAIT) cells in relapsing remitting (RRMS) and primary progressive MS (PPMS) patients Methods: Forty-five RRMS patients in remission, 20 RRMS patients experiencing exacerbations, 15 PPMS patients and 30 healthy controls (HCs) were included in the study. MAIT cells were identified phenotypically as CD3+ TCRγδ− Vα7.2 + CD161high. In 15 patients, MAIT cell number and MRI lesions were evaluated every 6 months, during 36 months. MAIT cell TCRVβ repertoire was defined using single cell cloning and mRNA sequencing. Results: Circulating MAIT cells were significantly reduced in both RRMS and PPMS patients, particularly during exacerbations, compared to healthy subjects. This decrease was accompanied by pro-inflammatory cytokine production (TNF-α, IFN-γ, IL-17 and GM-CSF). MAIT cells numbers were also lower during MS relapses. Three months post exacerbation, MAIT cell percentages increased significantly along with clinical recovery. Likewise, we observed inverse correlation between MRI lesions and MAIT cell numbers. In paired samples, MAIT cell percentage was significantly higher in CSF than peripheral blood. Finally, MAIT cells showed limited TCRVβ repertoires, in both CSF and peripheral blood, which remained stable over time. Conclusions: MAIT cell levels correlated with MS course both clinically and radiologically, showing marked and sustained oligoclonality. These findings may contribute to a better understanding of pathophysiological phenomena underlying the course of MS, and discovery of MAIT cell inhibitors could pave the way for the development of new therapeutic strategies. en_US
dc.language.iso eng en_US
dc.publisher Frontiers Research Foundation en_US
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Multiple Sclerosis en_US
dc.subject Esclerosis Múltiple en_US
dc.subject Cerebrospinal Fluid en_US
dc.subject Líquido Cefalorraquídeo en_US
dc.subject Mucosal-Associated Invariant T Cells en_US
dc.subject Células T Invariantes Asociadas a Mucosa en_US
dc.title Mucosal associated invariant T cell features and TCR repertoire characteristics during the course of Multiple Sclerosis en_US
dc.type info:eu-repo/semantics/publishedVersion
dc.type info:eu-repo/semantics/article en_US
dc.description.fil Fil: Carnero Contentti, Edgar. Hospital Alemán de Buenos Aires; Argentina.
dc.description.fil Fil: Farez, Mauricio Franco. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina.
dc.description.fil Fil: Correale, Jorge. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina.
dc.relation.ispartofCOUNTRY Suiza
dc.relation.ispartofCITY Lausana
dc.relation.ispartofTITLE Frontiers in immunology
dc.relation.ispartofISSN 1664-3224
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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