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Transferrin Enhances Neuronal Differentiation

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dc.contributor.author Pérez, María Julia
dc.contributor.author Carden, Tomás Roberto
dc.contributor.author Dos Santos Claro, Paula Ayelen
dc.contributor.author Silberstein, Susana
dc.contributor.author Páez, Pablo Martin
dc.contributor.author Cheli, Veronica Teresita
dc.contributor.author Correale, Jorge
dc.contributor.author Pasquini, Juana María
dc.date.accessioned 2024-07-11T14:19:32Z
dc.date.available 2024-07-11T14:19:32Z
dc.date.issued 2023-04-24
dc.identifier.citation Pérez MJ, Carden TR, Dos Santos Claro PA, Silberstein S, Páez PM, Cheli VT, Correale J, Pasquini JM. Transferrin Enhances Neuronal Differentiation. ASN Neuro. 2023 Jan-Dec;15:17590914231170703. doi: 10.1177/17590914231170703 es_ES
dc.identifier.uri https://doi.org/10.1177/17590914231170703
dc.identifier.uri https://repositorio.fleni.org.ar/xmlui/handle/123456789/1144
dc.description.abstract Although transferrin (Tf) is a glycoprotein best known for its role in iron delivery, iron-independent functions have also been reported. Here, we assessed apoTf (aTf) treatment effects on Neuro-2a (N2a) cells, a mouse neuroblastoma cell line which, once differentiated, shares many properties with neurons, including process outgrowth, expression of selective neuronal markers, and electrical activity. We first examined the binding of Tf to its receptor (TfR) in our model and verified that, like neurons, N2a cells can internalize Tf from the culture medium. Next, studies on neuronal developmental parameters showed that Tf increases N2a survival through a decrease in apoptosis. Additionally, Tf accelerated the morphological development of N2a cells by promoting neurite outgrowth. These pro-differentiating effects were also observed in primary cultures of mouse cortical neurons treated with aTf, as neurons matured at a higher rate than controls and showed a decrease in the expression of early neuronal markers. Further experiments in iron-enriched and iron-deficient media showed that Tf preserved its pro-differentiation properties in N2a cells, with results hinting at a modulatory role for iron. Moreover, N2a-microglia co-cultures revealed an increase in IL-10 upon aTf treatment, which may be thought to favor N2a differentiation. Taken together, these findings suggest that Tf reduces cell death and favors the neuronal differentiation process, thus making Tf a promising candidate to be used in regenerative strategies for neurodegenerative diseases. es_ES
dc.language.iso eng es_ES
dc.publisher Sage es_ES
dc.rights info:eu-repo/semantics/openAccess
dc.subject Cell Differentiation es_ES
dc.subject Diferenciación Celular es_ES
dc.subject Cell Line es_ES
dc.subject Línea Celular es_ES
dc.subject Iron es_ES
dc.subject Hierro es_ES
dc.subject Neurons es_ES
dc.subject Neuronas es_ES
dc.subject Transferrin es_ES
dc.subject Transferrina es_ES
dc.title Transferrin Enhances Neuronal Differentiation es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/publishedVersion
dc.description.fil Fil: Correale, Jorge. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina.
dc.relation.ispartofVOLUME 15
dc.relation.ispartofCOUNTRY Estados Unidos
dc.relation.ispartofCITY Thousand Oaks
dc.relation.ispartofTITLE ASN neuro
dc.relation.ispartofISSN 1759-0914
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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