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Ontogenetic Oligodendrocyte Maturation Through Gestational Iron Deprivation: The Road Not Taken

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dc.contributor.author Guitart, María Eugenia
dc.contributor.author Vence, Marianela
dc.contributor.author Correale, Jorge
dc.contributor.author Pasquini, Juana María
dc.contributor.author Rosato-Siri, María Victoria
dc.date.accessioned 2020-03-05T14:52:40Z
dc.date.available 2020-03-05T14:52:40Z
dc.date.issued 2019-06-04
dc.identifier.citation Guitart, M.E., Vence, M., Correale, J., Pasquini, J.M., Rosato-Siri, M.V. Ontogenetic oligodendrocyte maturation through gestational iron deprivation: The road not taken. Glia 2019 Sep;67(9):1760-1774. doi: 10.1002/glia.23647 en_US
dc.identifier.uri https://doi.org/10.1002/glia.23647
dc.identifier.uri https://repositorio.fleni.org.ar/handle/123456789/193
dc.description.abstract Developmental iron deficiency (dID) models facilitate the study of specific oligodendrocyte (OL) requirements for their progression to a mature state and subsequent contribution to myelination. In the current work, we used the dID model in transgenic mice expressing green fluorescence protein under the CNPase promoter allowing the identification of cells belonging to the oligodendroglial lineage, and the visualization of the entire myelin structure and single OL morphology. The present work evaluates dID effects on OL complexity in different brain areas. Control animals showed an increase in OL complexity both during development and along the anterior–posterior axis. In contrast, dID animals exhibited an initial increase in CNPase+ cells with prevalence of immature‐OL (i‐OL), an effect later compensated during development by selective death of those i‐OL. As a consequence, developmental behavior was impaired in terms of body balance, muscle response, and sensorimotor functions. To explore why i‐OL fail to mature in dID, expression levels of transcriptional factors involved in the maturation of the OL lineage were studied. In nuclear fractions, dID animals showed an increase in Hes5, which prevents the maturation of i‐OL, and a decrease in Sox10, a positive regulator of OL maturation. The cytoplasmic fractions showed a decrease in Olig1, which is critical for precursor cell differentiation into premyelinating OL. Overall, the expression levels of Hes5, Sox10, and Olig1 in dID conditions correlated with an unfavorable OL maturation profile. In sum, the current results provide further evidence of dID impact on myelination, keeping OL away from the maturational path. en_US
dc.language.iso eng en_US
dc.publisher Wiley en_US
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject Green Fluorescent Proteins en_US
dc.subject Proteínas Fluorescentes Verdes en_US
dc.subject Iron Metabolism Disorders en_US
dc.subject Trastornos del Metabolismo del Hierro en_US
dc.subject Oligodendroglia en_US
dc.subject Prenatal Nutritional Physiological Phenomena en_US
dc.subject Fenómenos Fisiológicos de la Nutrición Prenatal en_US
dc.title Ontogenetic Oligodendrocyte Maturation Through Gestational Iron Deprivation: The Road Not Taken en_US
dc.type info:eu-repo/semantics/publishedVersion
dc.type info:eu-repo/semantics/article en_US
dc.description.fil Fil: Guitart, María Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Instituto de Química y Fisicoquímica Biológicas Profesor "Alejandro C. Paladini"; Argentina.
dc.description.fil Fil: Vence, Marianela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Instituto de Química y Fisicoquímica Biológicas Profesor "Alejandro C. Paladini"; Argentina.
dc.description.fil Fil: Correale, Jorge. Fleni. Departamento de Neurología. Servicio de Neuroinmunología y Enfermedades Desmielinizantes; Argentina
dc.description.fil Fil: Pasquini, Juana María. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Instituto de Química y Fisicoquímica Biológicas Profesor "Alejandro C. Paladini"; Argentina.
dc.description.fil Fil: Rosato-Siri, María Victoria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Instituto de Química y Fisicoquímica Biológicas Profesor "Alejandro C. Paladini"; Argentina.
dc.relation.ispartofVOLUME 67
dc.relation.ispartofNUMBER 9
dc.relation.ispartofPAGINATION 1760-1774
dc.relation.ispartofCOUNTRY Estados Unidos
dc.relation.ispartofCITY Nueva York
dc.relation.ispartofISSN 1098-1136
dc.type.snrd info:ar-repo/semantics/artículo es_ES


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